The spirits I summoned, now I can’t get rid of them! 
                                                  W. Goethe, The Sorcerers Apprentice

This, the 6th edition of the  Tutorial, presents  an update, a  review and history of Flow Injection Techniques and  shows how much has changed,  since the first edition of this Tutorial was published in 1999. There are now over 20,000 papers and 23 monographs published on FIA and related techniques. Sequential Injection, Sequential Injection Chromatography and “Lab-On-Valve”  have been accepted by the scientific community, and widely applied to routine and industrial assays. In this Edition, the  reader will notice major changes in Chapter 2, where most recent advances in Sequential Injection, achieved by means of flow and temperature programming, are being presented for the first time. (2.2.12. and 2.6.1.)

Throughout the years the technology of scientific publishing, as we knew it, changed as well, for better, or worse. Printed scientific journals are now almost  extinct, we do not go to the library, instead we  go daily  on line. This change of technology allowed me to become publisher, editor, as well as the referee of this Tutorial. Such freedom in choice of scope, form, materials and viewpoints, was impossible within the framework of traditional scientific publishing. Free from the constraints of black and white print, the content of  the Tutorial is enhanced by colors and video images, and can be continuously updated. It is also available, free of charge worldwide, like some kind of mini Wikipedia.  The NEWS section is designed to bring  information on forthcoming meetings and publications, as well as to post your comments. Do not miss reading FIA STORY  a colorful quilt of tales, written  by leaders in the field, who generously share their views and experiences, of exploring  this exciting field of research and business opportunities.

On line publishing enhances Tutorial’s accessibility, and its format makes it convenient to use for research and teaching. As in the past, it will be a pleasure, to give permission to reproduce figures and material from this work, in Ph.D. theses, in presentations, and in all other non commercial publications. I am grateful to my friends, Elo Hansen and Andrew Starzynski for joining me in this endeavor, to Chris Measures for sharing his laboratory and views, and to Graham Marshall for building for me a prototype of the next generation of SI instrument.

Honolulu, November 2014

Jarda Ruzicka